Our laboratory has previously demonstrated that fluctuations in medium composition in multilayer cultures of epithelial tissues (epidermal and corneal epithelial tissues) with three-dimensional structures can be used to understand the state of the epithelial tissues. In addition, this experience is applied to suspension cultures of iPS cell aggregates for the mass production recently. In this presentation, I will introduce our techniques of medium analysis and their applications.

Masahiro Kino-Oka, Ph.D.
Professor in Department of Biotechnology& Director in Research Base for Cell Manufacturability, Graduate School of Engineering, Osaka University